regmedjournalРегенерация органов и тканейРегенерация органов и тканей2949-5938Общество регенеративной медицины10.60043/2949-5938-2024-3-41-55regmedjournal-79Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзменение внутриклеточной сигнализации, индуцированной норадреналином и серотонином, при старении мультипотентных мезенхимных стромальных клеток человекаChanges in noradrenaline- and serotonin-dependent intracellular signaling in senescent multipotent mesenchymal stromal cellsЧечехинаЕ. С.ChechekhinaElizaveta S.

Чечехина Елизавета Сергеевна — аспирант кафедры биохимии и регенеративной биомедицины Факультета фундаментальной медицины Медицинского научно-образовательного института,

119192, Москва, Ломоносовский пр., 27 к. 10.

Elizaveta S. Chechekhina — PhD student of Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Medical Research and Educational Institute,

119192, Moscow, Lomonosovsky ave., 27–10.

КулебякинК. Ю.KulebyakinKonstantin Yu.

Кулебякин Константин Юрьевич — к.б.н., доцент кафедры биохимии и регенеративной биомедицины Факультета фундаментальной медицины Медицинского научно-образовательного института; ведущий научный сотрудник лаборатории молекулярной эндокринологии Центра регенеративной медицины Медицинского научно-образовательного института,

119192, Москва, Ломоносовский пр., 27 к. 10.

 

Konstantin Yu. Kulebyakin — Dr. Sci. (Biology), Associate Professor of Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Medical Research and Education Institute; lead scientific fellow, laboratory of molecular endocrinology, Center for Regenerative Medicine, Medical Research and Education Institute,

119192, Moscow, Lomonosovsky ave., 27–10.

ГригорьеваО. А.GrigorievaOlga A.

Григорьева Ольга Александровна — к.б.н., старший научный сотрудник Научно-исследовательской лаборатории генных и клеточных технологий кафедры биохимии и регенеративной биомедицины Факультета фундаментальной медицины Медицинского научно-образовательного института; научный сотрудник лаборатории репарации и регенерации тканей Центра регенеративной медицины Медицинского научно-образовательного института,

119192, Москва, Ломоносовский пр., 27 к. 10.

 

Olga A. Grigorieva — Dr. Sci. (Biology), senior scientist of Laboratory of Gene and Cellular Biology, Faculty of Medicine, Medical Research and Educational Institute; scientist in Laboratory of Tissue Repair and Regeneration, Center for Regenerative Medicine, Medical Research and Educational Institute,

119192, Moscow, Lomonosovsky ave., 27–10.

ЕфименкоА. Ю.EfimenkoAnastasiya Yu.

Ефименко Анастасия Юрьевна — д.м.н., заведующая лабораторией репарации и регенерации тканей Центра регенеративной медицины Медицинского научно-образовательного института; доцент кафедры биохимии и регенеративной биомедицины Факультета фундаментальной медицины Медицинского научно-образовательного института,

119192, Москва, Ломоносовский пр., 27 к. 10.

Anastasiya Yu. Efimenko — MD, Dr. Sci. (Medicine), head of Laboratory of Tissue Repair and Regeneration, Center for Regenerative Medicine, Medical Research and Education Institute; Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Medical Research and Education Institute,

119192, Moscow, Lomonosovsky ave., 27–10.

Тюрин-КузьминП. А.Tyurin-KuzminPyotr A.

Тюрин-Кузьмин Петр Алексеевич — д.б.н., доцент, доцент кафедры биохимии и регенеративной биомедицины Факультета фундаментальной медицины Медицинского научно-образовательного института,

119192, Москва, Ломоносовский пр., 27 к. 10.

Pyotr A. Tyurin-Kuzmin — Dr. Sci. (Biology), Associate Professor of Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Medical Research and Educational Institute,

119192, Moscow, Lomonosovsky ave., 27–10.

tyurinkuzminpa@my.msu.ruФГБОУ ВО «Московский государственный университет имени М.В. Ломоносова»РоссияLomonosov Moscow State UniversityRussian Federation202413092025234155Copyright © Чечехина Е.С., Кулебякин К.Ю., Григорьева О.А., Ефименко А.Ю., Тюрин-Кузьмин П.А., 20252025Чечехина Е.С., Кулебякин К.Ю., Григорьева О.А., Ефименко А.Ю., Тюрин-Кузьмин П.А.Chechekhina E.S., Kulebyakin K.Y., Grigorieva O.A., Efimenko A.Y., Tyurin-Kuzmin P.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.regmed-journal.ru/jour/article/view/79

В данной работе мы исследовали изменения гормональной регуляции стволовых клеток жировой ткани человека при старении и то, как эти изменения ассоциированы с адипогенной дифференцировкой этих клеток. В качестве объекта изучения гормональной регуляции использовали постнатальные стволовые клетки жировой ткани — мультипотентные мезенхимные стромальные клетки (МСК). Мы показали, что как МСК с индуцированным репликативным старением, так и МСК, полученные от пожилых доноров, обладают сниженным адипогенным потенциалом, также у этих клеток нарушены механизмы регуляции адипогенной дифференцировки при действии норадреналина и серотонина. Изучение внутриклеточных сигнальных каскадов позволило установить, что при старении в МСК проявляется пониженная активация как цАМФ-зависимых, так и фосфоинозитид/ кальций-зависимых сигнальных каскадов. Кальциевые ответы на стимуляцию норадреналином и серотонином оказались отложенными во времени в МСК с индуцированным репликативным старением. Таким образом, старение приводит к снижению регуляторного воздействия гормонов-регуляторов на адипогенную дифференцировку МСК человека.

In this work, we studied how hormonal regulation of human adipose tissue stem cells changes during aging and how changes in hormonal regulation are associated with adipogenic differentiation of these cells. Postnatal adipose tissue stem cells — multipotent mesenchymal stromal cells (MSCs), were used as an object for studying hormonal regulation. We showed that both MSCs with induced replicative senescence and MSCs obtained from elderly donors have a reduced adipogenic potential. These cells have impaired mechanisms of regulation of adipogenic differentiation under noradrenaline and serotonin. The study of intracellular signaling cascades allowed us to establish that during senescence, MSCs exhibit reduced activation of both cAMP-dependent and phosphoinositide/calcium-dependent signaling cascades. Moreover, calcium responses to the addition of these hormones were delayed in time in MSCs with induced replicative aging. Thus, senescence leads to a decrease in the regulatory effect of hormones on the adipogenic differentiation of human MSCs.

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The authors declare that there are no conflicts of interest present.