The article describes the prerequisites and experience of creation, as well as the current stage of development of the new scientific journal “Tissue and Organ Regeneration”, determines its place in the publication landscape in Russia, justifies the need to develop a program for the development of the journal as a necessary stage for improving the quality of the content of the journal and its representation in the academic community.

The implementation of the proposed program, in our opinion, will help optimize and increase the efficiency of the editorial board’s activities for the further scientific development of journal, and in the future will allow it to be included in Russian and international scientometric databases.

The aim of this review was to describe and compare molecular cloning methods for assembling genetic constructs. Genetic therapy is one of the rapidly developing fields of modern medicine, so special attention in this review is paid to parameters such as speed, accuracy, and efficiency of cloning, as these are critical factors in creating gene therapy agents. Special attention is given to the Golden Gate method, which is based on the use of type IIS restriction endonucleases, as this approach simplifies the cloning process and increases its efficiency through standardized design and a minimal set of enzymes. Alongside Golden Gate, the review also discusses more innovative molecular cloning methods, Gateway and Gibson, in terms of their potential use for addressing fundamental and applied challenges in regenerative medicine.

Modern regenerative medicine widely uses collagen-based products and products, the most popular being collagen of animal origin. The use of such collagen is associated with a number of difficulties, incl. with the occurrence of active immune reactions, as well as with religious and cultural restrictions that do not allow the use of drugs made from animal tissue. Marine collagen, which does not have these disadvantages, can be a successful alternative to collagen from animal sources. The current geopolitical situation forces us to look for domestic sources of collagen. The article provides an overview of methods for extracting collagen from jellyfish biomass. Promising species of jellyfish suitable for implementing these methods are considered. It has been shown that on the territory of the Russian Federation, the most productive and promising jellyfish of the family Rhizostomatidae of the order of Rhizostoma pulmo, living in the Black Sea. The jellyfish Aurelia aurita of the order of disc jellyfish (Semaeostomeae), despite its prevalence (lives in almost all seas of Russia), has a low content of dry substances in its biomass and is difficult to catch due to the small weight of individuals. Of the extraction methods considered in relation to jellyfish biomass, mixed enzymatic and acidic extraction using organic acids should be considered the most suitable. The enzymatic method using pepsin is applicable to the biomass of Aurelia aurita jellyfish, because The jellyfish biomass is completely dissolved in the first stage of enzymatic extraction.

The article presents lecture materials on regeneration mechanisms from the textbook “Lectures on Regenerative Medicine”. An example of epimorphic regeneration in mammals is considered — the cellular mechanisms of tissue restoration of the fingertip after amputation. This model is a relevant object of research, since it allows us to study the mechanisms of both the formation of a specialized temporary structure of the blastema and subsequent morphogenesis, which ends with the full restoration of all amputated tissues.

Genome editing technologies and their modifications are an indispensable tool for studying the functions of individual molecules, obtaining cell lines and animals with specified properties, and developing promising approaches to the therapy of previously untreatable diseases. This review covers various aspects of genome editing technologies: from their biological significance to the principles of their functioning and the most promising areas of application in basic and applied research. Particular attention is paid to discussing the limitations of genome editing technologies, as well as the legal and ethical aspects of their application to human genome modification. This review may be of interest to a wide range of readers, including researchers wishing to learn more about genome editing technologies and planning their practical application.