Golden gate method in biology and medicine
https://doi.org/10.60043/2949-5938-2024-1-16-28
Abstract
The aim of this review was to describe and compare molecular cloning methods for assembling genetic constructs. Genetic therapy is one of the rapidly developing fields of modern medicine, so special attention in this review is paid to parameters such as speed, accuracy, and efficiency of cloning, as these are critical factors in creating gene therapy agents. Special attention is given to the Golden Gate method, which is based on the use of type IIS restriction endonucleases, as this approach simplifies the cloning process and increases its efficiency through standardized design and a minimal set of enzymes. Alongside Golden Gate, the review also discusses more innovative molecular cloning methods, Gateway and Gibson, in terms of their potential use for addressing fundamental and applied challenges in regenerative medicine.
Supporting agencies: This research was supported from the Russian Federal Academic Leadership Program Priority 2030 at the Immanuel Kant Baltic Federal University.
About the Authors
M. I. Antipina
Immanuel Kant Baltic Federal University
Russian Federation
Russian Federation
Maria I. Antipina — Research Engineer at the Translational Research Laboratory of the Institute of Medicine and Life Sciences (MEDBIO)
Alexander Nevsky Str., 14, 236041, Kaliningrad
V. A. Li
Immanuel Kant Baltic Federal University
Russian Federation
Russian Federation
Vladislav A. Li — 4th-year student specializing in Bioengineering and Bioinformatics at the Institute of Medicine and Life Sciences (MEDBIO)
Alexander Nevsky Str., 14, 236041, Kaliningrad
E. E. Popova
Immanuel Kant Baltic Federal University
Russian Federation
Russian Federation
Elizaveta E. Popova — 4th-year student specializing in Bioengineering and Bioinformatics at the Institute of Medicine and Life Sciences (MEDBIO)
Alexander Nevsky Str., 14, 236041, Kaliningrad
E. V. Semina
Immanuel Kant Baltic Federal University
Russian Federation
Russian Federation
Ekaterina V. Semina — Dr. Sci. (Biology), Head of the Translational Research Laboratory at the Institute of Medicine and Life Sciences (MEDBIO)
Alexander Nevsky Str., 14, 236041, Kaliningrad
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Review
For citations:
Antipina M.I., Li V.A., Popova E.E., Semina E.V. Golden gate method in biology and medicine. Регенерация органов и тканей. 2024;2(1):16-28. (In Russ.) https://doi.org/10.60043/2949-5938-2024-1-16-28
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